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Simvastatin-Cy5.5, pravastatin-Cy5.5, and free Cy5.5 uptake was measured by fluorescence (red) in (a) Panc1 ( KRAS MUT ) and <t>BxPC3</t> ( KRAS WT ) and (b) HPDE KRAS MUT (HPDE i KRAS with doxycycline) and HPDE KRAS WT (HPDE i KRAS without doxycycline). The scale bars indicate 10 μm. The cell nuclei were stained with DAPI (blue). Quantitative measurements of the cellular uptake of the statin-dye conjugates are presented relative fluorescence intensity per cell count. Bars indicate Mean ± S.E. (n ≥ 3). Statistically significant differences are represented as * for p < 0.05, *** for p < 0.001, and **** for p < 0.0001.
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Simvastatin-Cy5.5, pravastatin-Cy5.5, and free Cy5.5 uptake was measured by fluorescence (red) in (a) Panc1 ( KRAS MUT ) and <t>BxPC3</t> ( KRAS WT ) and (b) HPDE KRAS MUT (HPDE i KRAS with doxycycline) and HPDE KRAS WT (HPDE i KRAS without doxycycline). The scale bars indicate 10 μm. The cell nuclei were stained with DAPI (blue). Quantitative measurements of the cellular uptake of the statin-dye conjugates are presented relative fluorescence intensity per cell count. Bars indicate Mean ± S.E. (n ≥ 3). Statistically significant differences are represented as * for p < 0.05, *** for p < 0.001, and **** for p < 0.0001.
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pancreatic cell line bxpc3  (ATCC)
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Simvastatin-Cy5.5, pravastatin-Cy5.5, and free Cy5.5 uptake was measured by fluorescence (red) in (a) Panc1 ( KRAS MUT ) and <t>BxPC3</t> ( KRAS WT ) and (b) HPDE KRAS MUT (HPDE i KRAS with doxycycline) and HPDE KRAS WT (HPDE i KRAS without doxycycline). The scale bars indicate 10 μm. The cell nuclei were stained with DAPI (blue). Quantitative measurements of the cellular uptake of the statin-dye conjugates are presented relative fluorescence intensity per cell count. Bars indicate Mean ± S.E. (n ≥ 3). Statistically significant differences are represented as * for p < 0.05, *** for p < 0.001, and **** for p < 0.0001.
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Simvastatin-Cy5.5, pravastatin-Cy5.5, and free Cy5.5 uptake was measured by fluorescence (red) in (a) Panc1 ( KRAS MUT ) and <t>BxPC3</t> ( KRAS WT ) and (b) HPDE KRAS MUT (HPDE i KRAS with doxycycline) and HPDE KRAS WT (HPDE i KRAS without doxycycline). The scale bars indicate 10 μm. The cell nuclei were stained with DAPI (blue). Quantitative measurements of the cellular uptake of the statin-dye conjugates are presented relative fluorescence intensity per cell count. Bars indicate Mean ± S.E. (n ≥ 3). Statistically significant differences are represented as * for p < 0.05, *** for p < 0.001, and **** for p < 0.0001.
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Simvastatin-Cy5.5, pravastatin-Cy5.5, and free Cy5.5 uptake was measured by fluorescence (red) in (a) Panc1 ( KRAS MUT ) and BxPC3 ( KRAS WT ) and (b) HPDE KRAS MUT (HPDE i KRAS with doxycycline) and HPDE KRAS WT (HPDE i KRAS without doxycycline). The scale bars indicate 10 μm. The cell nuclei were stained with DAPI (blue). Quantitative measurements of the cellular uptake of the statin-dye conjugates are presented relative fluorescence intensity per cell count. Bars indicate Mean ± S.E. (n ≥ 3). Statistically significant differences are represented as * for p < 0.05, *** for p < 0.001, and **** for p < 0.0001.

Journal: PLOS One

Article Title: Statin-dye conjugates for selective targeting of KRAS mutant cancer cells

doi: 10.1371/journal.pone.0340189

Figure Lengend Snippet: Simvastatin-Cy5.5, pravastatin-Cy5.5, and free Cy5.5 uptake was measured by fluorescence (red) in (a) Panc1 ( KRAS MUT ) and BxPC3 ( KRAS WT ) and (b) HPDE KRAS MUT (HPDE i KRAS with doxycycline) and HPDE KRAS WT (HPDE i KRAS without doxycycline). The scale bars indicate 10 μm. The cell nuclei were stained with DAPI (blue). Quantitative measurements of the cellular uptake of the statin-dye conjugates are presented relative fluorescence intensity per cell count. Bars indicate Mean ± S.E. (n ≥ 3). Statistically significant differences are represented as * for p < 0.05, *** for p < 0.001, and **** for p < 0.0001.

Article Snippet: Panc1 (CRL-1469) and BxPC3 (CRL-1687) cell lines were purchased from the American Type Culture Collection.

Techniques: Fluorescence, Staining, Cell Counting